| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Tat, Thomas A (14)
|
GUSHAC |
24.18 |
24.41 |
0.23 |
| 2 |
Chen, Daniel J (13)
|
GUSWAT |
26.52 |
24.53 |
-1.99 |
| 3 |
Nuval, Mack A (13)
|
GUUN-S |
27.14 |
24.90 |
-2.24 |
| 4 |
Munoz, Angel (14)
|
GUHCAP |
25.73 |
25.18 |
-0.55 |
| 5 |
McDonald, Christopher R (14)
|
GUSHAC |
27.64 |
25.98 |
-1.66 |
| 6 |
Broughton, Jeremiah A (13)
|
GUHCAP |
26.23 |
26.14 |
-0.09 |
| 7 |
Smith, Carson T (13)
|
GUSHAC |
28.31 |
26.55 |
-1.76 |
| 7 |
Shkarbul, Daniil Y (13)
|
GUCOOG |
31.04 |
26.55 |
-4.49 |
| 9 |
Linsenmayer, David H (13)
|
GUCOOG |
27.02 |
26.65 |
-0.37 |
| 10 |
Marlin, Solomon D (14)
|
GUHCAP |
31.78 |
26.66 |
-5.12 |
| 11 |
Charles, David L (13)
|
GUHCAP |
27.65 |
27.11 |
-0.54 |
| 12 |
Suvalic, Davud (14)
|
GUCOOG |
31.62 |
27.37 |
-4.25 |
| 13 |
Ulrich, Cooper E (14)
|
GUTTST |
31.34 |
27.38 |
-3.96 |
| 14 |
Arismendez-Reyes, Lorenzo I (13)
|
GUSHAC |
27.48 |
27.44 |
-0.04 |
| 15 |
Soto, Efrain A (13)
|
GUCOOG |
31.85 |
27.62 |
-4.23 |
| 16 |
Orellana, Alexander (14)
|
GUHCAP |
29.01 |
28.31 |
-0.70 |
| 17 |
Miles, Elliott B (13)
|
GUAQUA |
28.94 |
28.37 |
-0.57 |
| 18 |
Joiner, Nathaniel W (13)
|
GUAQUA |
27.89 |
28.63 |
0.74 |
| 19 |
Huang, Haoxuan (13)
|
GUHCAP |
30.42 |
29.11 |
-1.31 |
| 20 |
Shaw, Ethan A (13)
|
GULAP |
29.58 |
29.40 |
-0.18 |
| 21 |
Ervesun, Eduardo M (13)
|
GUAQUA |
31.24 |
29.67 |
-1.57 |
| 22 |
Szklaruk-Traipe, David I (14)
|
GUSHRK |
36.91 |
29.76 |
-7.15 |
| 23 |
Hoffman, Cade C (13)
|
GUSHAC |
40.73 |
30.20 |
-10.53 |
| 24 |
Song, Eric Z (14)
|
GUSWAT |
|
30.24 |
- |
| 25 |
LeCompte, Luke H (13)
|
GUSHRK |
34.09 |
30.28 |
-3.81 |
| 26 |
Charles, Noah B (13)
|
GUHCAP |
30.85 |
30.31 |
-0.54 |
| 27 |
Bautista, Jared I (13)
|
GUHCAP |
30.95 |
30.96 |
0.01 |
| 28 |
Martinez, Luis S (13)
|
GUHCAP |
32.85 |
31.11 |
-1.74 |
| 29 |
Rodriguez, Pedro E (14)
|
GUHCAP |
34.31 |
31.76 |
-2.55 |
| 30 |
Blanco, Joshua A (14)
|
GUUN-S |
|
32.31 |
- |
| 31 |
Grabein, Aiden K (13)
|
GUSHAC |
|
36.22 |
- |