| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Opitz, Lillian K (7)
|
AMPEAQ |
22.72 |
20.75 |
-1.97 |
| 2 |
Hull, Naomi D (7)
|
AMFAST |
24.73 |
22.20 |
-2.53 |
| 3 |
Rees, Leia K (7)
|
AMNHA |
27.53 |
26.41 |
-1.12 |
| 4 |
Logan, Mackenzie E (7)
|
AMSASC |
28.40 |
26.62 |
-1.78 |
| 5 |
Hunsinger, Sadie G (7)
|
AMFAST |
28.48 |
31.09 |
2.61 |
| 6 |
Radomski, Annabelle M (7)
|
AMHD |
36.22 |
34.13 |
-2.09 |
|
Berke, Mikayla R (7)
|
AMHD |
44.98 |
DQ |
- |
|
Cegan, Olivia R (7)
|
AMHD |
33.89 |
DQ |
- |
|
Page, Brynley J (7)
|
AMHD |
38.98 |
DQ |
- |
|
Gatto, Brooke M (7)
|
AMMLAC |
25.99 |
DQ |
- |
|
Stager, Nora J (7)
|
MANLAC |
29.30 |
DQ |
- |
|
Hollenbeck, Mackenzie E (7)
|
AMANSC |
26.02 |
NS |
- |
|
Wood, Beatrice G (7)
|
AMKW |
33.02 |
NS |
- |
|
Krauze, Ana I (7)
|
AMJCCS |
50.20 |
NS |
- |