| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guan, Fiona (8)
|
AMANSC |
40.11 |
40.23 |
0.12 |
| 2 |
Dietiker, Claire L (8)
|
AMPTSC |
43.44 |
43.65 |
0.21 |
| 3 |
Susko, Caitlin A (8)
|
AMMMSA |
50.29 |
48.60 |
-1.69 |
| 4 |
Turner, Alyssa J (8)
|
AMRACE |
53.21 |
50.10 |
-3.11 |
| 5 |
Jackson, Alanna R (8)
|
AMWAAC |
58.49 |
54.05 |
-4.44 |
| 6 |
Clark, Riley M (8)
|
AMSAAT |
46.66 |
59.00 |
12.34 |
| 7 |
Dudkowski, Alina G (8)
|
AMPRA |
56.91 |
59.16 |
2.25 |
| 8 |
Dubowitz, Maayan O (8)
|
AMJCCS |
1:10.20 |
1:04.70 |
-5.50 |
|
Sunderlin, Charlotte R (8)
|
AMANSC |
59.26 |
DQ |
- |
|
Starz, Rian O (8)
|
AMKW |
53.76 |
DQ |
- |
|
Alexander, Annabelle E (8)
|
AMSVSC |
1:39.92 |
DQ |
- |
|
Marks, Ariana R (8)
|
AMSVSC |
57.04 |
DQ |
- |