| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Corcoran, Levi D (9)
|
MAMAKO |
31.58 |
30.72 |
-0.86 |
| 2 |
Kurdes, James K (9)
|
MAMAKO |
31.10 |
30.97 |
-0.13 |
| 3 |
Evans, Connor W (9)
|
MAMAKO |
32.75 |
32.17 |
-0.58 |
| 4 |
Shockley, Finneas S (10)
|
MDHFY |
35.22 |
35.91 |
0.69 |
| 5 |
McCullough, Tristan M (9)
|
MAMAKO |
|
36.33 |
- |
| 6 |
Dufrense, Caleb (10)
|
MADYD |
36.91 |
38.51 |
1.60 |
| 7 |
Auguste, Christian (10)
|
MADYD |
37.65 |
38.67 |
1.02 |
| 8 |
Gupta, Nidhish (10)
|
MDOPST |
38.53 |
38.71 |
0.18 |
| 9 |
Wright, Everton C (10)
|
MADYD |
37.52 |
39.35 |
1.83 |
| 10 |
Kraeuter, Paul F (9)
|
MDOPST |
39.99 |
39.38 |
-0.61 |
| 11 |
Jin, Peter (10)
|
MDHFY |
45.09 |
42.53 |
-2.56 |
| 12 |
St Pierre, Liam P (10)
|
MDOPST |
41.68 |
43.61 |
1.93 |
| 13 |
Swift, Alex T (10)
|
MDHFY |
45.35 |
45.72 |
0.37 |
| 14 |
Hinman, James A (10)
|
MDHFY |
43.55 |
46.04 |
2.49 |
| 15 |
Kim, Minsuk (10)
|
MDHFY |
52.79 |
46.23 |
-6.56 |
| 16 |
Folderauer, Bram (9)
|
MDHFY |
44.68 |
47.47 |
2.79 |
| 17 |
Dalfonso, Gabriel R (10)
|
MDOPST |
|
52.13 |
- |
| 18 |
Moore, Graham (9)
|
MDHFY |
55.08 |
1:05.46 |
10.38 |
|
Arvi, Nathaniel (9)
|
MDHFY |
43.31 |
NS |
- |
|
MacBlain, Gavin J (9)
|
MASFY |
1:12.24 |
NS |
- |