| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DiMaio, Lyla A (10)
|
AMRGSY |
2:15.16 |
2:21.91 |
6.75 |
| 2 |
Strock, Hannah J (10)
|
AMBPR |
2:26.23 |
2:27.61 |
1.38 |
| 3 |
Turner, Alyssa J (10)
|
AM3RVS |
2:35.30 |
2:34.51 |
-0.79 |
| 4 |
Webb, Marie C (10)
|
AMRGSY |
2:37.92 |
2:39.60 |
1.68 |
| 5 |
Namey, Evelynn A (10)
|
AMRXA |
2:42.99 |
2:44.31 |
1.32 |
| 6 |
Hodgson, Tatum J (10)
|
AMHD |
3:00.98 |
2:44.34 |
-16.64 |
| 7 |
Brooks, Hannah C (10)
|
AMSASC |
2:52.10 |
2:45.80 |
-6.30 |
| 8 |
Poltinnikov, Emily E (10)
|
AMFAST |
2:47.80 |
2:49.61 |
1.81 |
| 9 |
Watkins, Hannah E (9)
|
AMBPR |
2:58.22 |
2:49.98 |
-8.24 |
| 10 |
Lawrence, Rory C (9)
|
AMNHA |
2:59.31 |
2:54.52 |
-4.79 |
| 11 |
McAtee, Mikayla N (10)
|
AMNHA |
3:02.16 |
3:01.44 |
-0.72 |
| 12 |
Riley, Catherine E (10)
|
AMHD |
3:03.60 |
3:07.00 |
3.40 |
| 13 |
Good, Annalise C (10)
|
AMHD |
3:18.22 |
3:11.58 |
-6.64 |
| 14 |
Ricketts, Brenna E (10)
|
AMHD |
3:15.98 |
3:22.61 |
6.63 |
| 15 |
Radomski, Annabelle M (9)
|
AMHD |
3:17.98 |
3:24.56 |
6.58 |
| 16 |
Henson, Jessica M (9)
|
AMBPR |
3:18.51 |
3:24.73 |
6.22 |
| 17 |
Cegan, Olivia R (9)
|
AMHD |
3:17.98 |
3:27.15 |
9.17 |
| 18 |
Van De Velde, Elle J (10)
|
AMHD |
3:17.98 |
3:29.96 |
11.98 |