| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dvorak, Mia I (14)
|
MDACA |
54.74 |
56.62 |
1.88 |
| 2 |
Dernetz, Izzy (14)
|
MDCGA |
55.54 |
56.66 |
1.12 |
| 3 |
Helminiak, Lauren M (13)
|
MDMAS |
54.28 |
57.92 |
3.64 |
| 4 |
Walker, Lydia K (14)
|
MDMAS |
1:01.00 |
58.55 |
-2.45 |
| 5 |
Pierce, Laci M (13)
|
MDACA |
59.48 |
1:00.23 |
0.75 |
| 6 |
Given, Caitlyn E (13)
|
MDSPRC |
1:02.48 |
1:01.38 |
-1.10 |
| 7 |
Graham, Rilee E (13)
|
MDACA |
58.81 |
1:01.45 |
2.64 |
| 8 |
Taylor, Lily M (14)
|
MDCGA |
1:01.85 |
1:02.37 |
0.52 |
| 9 |
Dworkin, Grace (14)
|
MDMSC |
1:02.99 |
1:02.64 |
-0.35 |
| 10 |
Fatkin, Emily G (14)
|
MDHSST |
1:06.11 |
1:04.44 |
-1.67 |
| 11 |
Fredericks, Trinity M (13)
|
MDMASU |
1:08.52 |
1:05.12 |
-3.40 |
| 12 |
Brandenburg, Grace H (14)
|
MDCGA |
1:06.04 |
1:06.72 |
0.68 |
| 13 |
Carney, Sara H (14)
|
MDMAS |
1:05.01 |
1:07.09 |
2.08 |
| 14 |
DeGuzman, Arianne Y (14)
|
MDMSC |
1:03.44 |
1:07.28 |
3.84 |
| 15 |
Aldana, Isabel E (13)
|
MDMAS |
1:07.75 |
1:07.30 |
-0.45 |
| 16 |
Van Gaal, Anna (14)
|
MDSPRC |
1:10.16 |
1:07.46 |
-2.70 |
| 17 |
Rogers, Kyla M (14)
|
MDMSC |
1:07.38 |
1:07.94 |
0.56 |
| 18 |
Payne, Mackenzie M (13)
|
MDHSST |
1:10.58 |
1:08.15 |
-2.43 |
| 19 |
O'Hare, Riley M (14)
|
MDHSST |
1:09.98 |
1:08.45 |
-1.53 |
| 20 |
Shapiro, Sara I (13)
|
MDJCC |
1:10.77 |
1:08.89 |
-1.88 |
| 21 |
Fixler, Alisa (13)
|
MDMSC |
1:08.69 |
1:09.00 |
0.31 |
| 22 |
Mroz, Maja (13)
|
MDACA |
1:10.79 |
1:09.53 |
-1.26 |
| 23 |
Reker, Emma E (13)
|
MDACA |
1:09.01 |
1:09.82 |
0.81 |
| 24 |
Williams, Zoe (14)
|
MDCGA |
1:16.24 |
1:16.10 |
-0.14 |
| 25 |
Chronister, Hannah B (13)
|
MDHSST |
1:18.58 |
1:17.55 |
-1.03 |
|
Kinsey, Lila N (13)
|
MDCGA |
1:03.59 |
NS |
- |