| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Seifert, Elizabeth A (8)
|
MDACA |
44.34 |
42.72 |
-1.62 |
| 2 |
Polish, Teagan R (8)
|
MDSPRC |
49.32 |
46.58 |
-2.74 |
| 3 |
Sasaki, Kumiko (7)
|
MDNBAC |
|
46.87 |
- |
| 4 |
Jones, Emily B (8)
|
MDYCM |
|
46.99 |
- |
| 5 |
Barowski, Isabel R (8)
|
MDACA |
46.35 |
48.56 |
2.21 |
| 6 |
Schaffer, Sakineh C (8)
|
MDMAS |
|
49.46 |
- |
| 7 |
Epp, Sandy L (7)
|
MDRAC |
51.75 |
49.66 |
-2.09 |
| 8 |
Stephan, Ainsley M (8)
|
MDEST |
44.51 |
50.00 |
5.49 |
| 9 |
Sheinin, Lyla J (7)
|
MDMAS |
1:02.52 |
50.74 |
-11.78 |
| 10 |
Gordy, Julia C (8)
|
MDFAST |
|
51.18 |
- |
| 11 |
Tyberg, Ellie A (7)
|
MDNBAC |
|
51.36 |
- |
| 12 |
Brissett, Gabrielle S (7)
|
MDEST |
|
51.72 |
- |
| 13 |
Spraul, Ella G (7)
|
MDSPRC |
|
51.96 |
- |
| 14 |
Hankins, Camryn L (8)
|
MDEST |
|
52.05 |
- |
| 15 |
Missry, Victoria A (8)
|
MDMAS |
53.93 |
53.06 |
-0.87 |
| 16 |
Chaudhuri, Isha (8)
|
MDRAC |
|
53.92 |
- |
| 17 |
Virmani, Marella A (8)
|
MDCAA |
|
54.38 |
- |
| 18 |
Labellarte, Viviana G (8)
|
MDMSC |
|
55.27 |
- |
| 19 |
Possidente, Lucia J (8)
|
MDMSC |
1:03.04 |
55.30 |
-7.74 |
| 20 |
Mahoney, Addison E (8)
|
MDMAS |
|
55.70 |
- |
| 21 |
Virmani, Lauren A (8)
|
MDCAA |
|
57.11 |
- |
| 22 |
Chew, Sophia M (6)
|
MDRAC |
|
58.63 |
- |
| 23 |
Brashear, Johanna H (8)
|
MDFAST |
1:05.02 |
1:01.50 |
-3.52 |
| 24 |
Rutz, Charlotte M (7)
|
MDYCM |
|
1:01.55 |
- |
| 25 |
Jackson, Zoe A (8)
|
MDNBAC |
|
1:09.80 |
- |
| 26 |
McCartney, Mallory A (8)
|
MDHSST |
|
1:19.14 |
- |
|
Anisiobi, Calia C (7)
|
MDNBAC |
|
DQ |
- |
|
Gleeson, Faraby C (8)
|
MDYCM |
|
DQ |
- |
|
Ripple, Reese L (7)
|
MDFSC |
1:09.04 |
DQ |
- |
|
Stevens, Elizabeth E (8)
|
MDYCM |
|
DQ |
- |