| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Fisk, Holly E (11)
|
NIFAST |
|
40.86 |
- |
| 2 |
Hartley, Elliana G (8)
|
NIFAST |
43.68 |
41.50 |
-2.18 |
| 3 |
Huerta-Andrade, Isabella (10)
|
NIFAST |
41.97 |
42.21 |
0.24 |
| 4 |
Black, Kaitlin (11)
|
NIFAST |
|
43.59 |
- |
| 5 |
Worrall, Zachary L (9)
|
NIFAST |
|
44.27 |
- |
| 6 |
Smith, Lauryn T (10)
|
NIFAST |
47.51 |
46.02 |
-1.49 |
| 7 |
Roeser, Connor K (10)
|
NIFAST |
|
48.25 |
- |
| 8 |
Roeser, Corey M (8)
|
NIFAST |
|
50.03 |
- |
| 9 |
Sotgiu, Isabella V (12)
|
NIFAST |
|
50.27 |
- |
| 10 |
Sousa, Evan F (9)
|
NIFAST |
48.58 |
50.69 |
2.11 |
| 11 |
Hanuch, Cecilia R (11)
|
NIFAST |
|
50.85 |
- |
| 12 |
Beach, Jadyn E (9)
|
NIFAST |
52.99 |
51.40 |
-1.59 |
| 13 |
Larzelere, Charlotte G (7)
|
NIFAST |
|
52.69 |
- |
| 14 |
Vadney, Sage E (11)
|
NIFAST |
52.30 |
53.06 |
0.76 |
| 15 |
Kalbfus, Taylor R (9)
|
NIFAST |
|
56.16 |
- |
| 16 |
Cougle, Adam J (11)
|
NIFAST |
|
57.44 |
- |
| 17 |
Huerta-Andrade, Amanda (9)
|
NIFAST |
1:00.48 |
57.67 |
-2.81 |
| 18 |
Majewski, Haley M (9)
|
NIFAST |
1:00.34 |
58.20 |
-2.14 |
| 19 |
Claus, Alivia M (12)
|
NIFAST |
|
58.59 |
- |
| 20 |
Conley, Michael T (11)
|
NIFAST |
|
1:00.08 |
- |
| 21 |
Oleyourryk, Caroline R (9)
|
NIFAST |
|
1:00.38 |
- |
| 22 |
Short, Grace T (10)
|
NIFAST |
|
1:01.66 |
- |
| 23 |
Schaeber, Emerson M (8)
|
NIFAST |
|
1:03.58 |
- |
| 24 |
Togay, Erdem A (9)
|
NIFAST |
|
1:15.50 |
- |
| 25 |
Brett, Catherine M (7)
|
NIFAST |
|
1:20.58 |
- |