| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Obuhanych, Zachary J (11)
|
NIFAST |
35.02 |
35.44 |
0.42 |
| 2 |
Black, Meghan C (12)
|
NIFAST |
39.78 |
39.02 |
-0.76 |
| 3 |
Huerta-Andrade, Isabella (11)
|
NIFAST |
40.74 |
41.23 |
0.49 |
| 4 |
Carter, Owen T (10)
|
NIFAST |
51.68 |
42.59 |
-9.09 |
| 5 |
Lindquist, Matthew J (11)
|
NIFAST |
|
45.85 |
- |
| 6 |
Larzelere, Natalie E (9)
|
NIFAST |
42.93 |
48.65 |
5.72 |
| 7 |
Hagen, Nicolette E (11)
|
NIFAST |
47.04 |
49.08 |
2.04 |
| 8 |
Vadney, Sage E (11)
|
NIFAST |
49.12 |
50.21 |
1.09 |
| 9 |
Montaque, Angelina M (10)
|
NIFAST |
52.46 |
54.52 |
2.06 |
| 10 |
Morgano, Helena G (10)
|
NIFAST |
57.43 |
59.19 |
1.76 |
| 11 |
Oleyourryk, Caroline R (9)
|
NIFAST |
1:00.38 |
1:02.44 |
2.06 |
|
Balland, Megan E (9)
|
NIFAST |
52.25 |
DQ |
- |
|
Fisk, Holly E (12)
|
NIFAST |
39.45 |
NS |
- |
|
Galley, Alyssa E (9)
|
NIFAST |
51.28 |
NS |
- |