| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Carter, Owen T (11)
|
NIFAST |
41.32 |
37.72 |
-3.60 |
| 2 |
Little, Mikayla L (11)
|
NIFAST |
45.33 |
43.31 |
-2.02 |
| 3 |
Lawrence, Kaleigh M (9)
|
NIFAST |
43.54 |
43.40 |
-0.14 |
| 4 |
Wang, Sophia Y (9)
|
NIFAST |
44.20 |
45.02 |
0.82 |
| 5 |
Galley, Alyssa E (10)
|
NIFAST |
45.49 |
46.46 |
0.97 |
| 6 |
Laurence, Bridget K (10)
|
NIFAST |
44.67 |
47.64 |
2.97 |
| 7 |
Preston, Evan W (11)
|
NIFAST |
43.83 |
48.22 |
4.39 |
| 8 |
Nacca, Giada F (9)
|
NIFAST |
48.22 |
49.21 |
0.99 |
| 9 |
Rusho, Hannah F (9)
|
NIFAST |
49.65 |
49.25 |
-0.40 |
| 10 |
Ferrari, Abigail C (10)
|
NIFAST |
|
49.32 |
- |
| 11 |
Cougle, Adam J (12)
|
NIFAST |
51.37 |
50.77 |
-0.60 |
| 12 |
Ogletree, Blaine A (10)
|
NIFAST |
52.18 |
52.89 |
0.71 |
| 13 |
Blanchard, Zachary E (11)
|
NIFAST |
49.60 |
53.69 |
4.09 |
| 14 |
Comstra, Connor D (9)
|
NIFAST |
1:00.93 |
55.39 |
-5.54 |
| 15 |
Schwartzbeck, Elisabeth M (11)
|
NIFAST |
|
57.45 |
- |
| 16 |
Donnaruma, Logan A (9)
|
NIFAST |
1:03.34 |
58.33 |
-5.01 |
| 17 |
Casella, Isabelle R (9)
|
NIFAST |
|
1:07.75 |
- |
|
Larzelere, Charlotte G (8)
|
NIFAST |
48.00 |
DQ |
- |
|
Webb, Karen R (11)
|
NIFAST |
46.78 |
DQ |
- |