| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCauley, Angela E (17)
|
ADEELS |
4:55.99 |
4:57.22 |
1.23 |
| 2 |
Felton, Eleanor V (16)
|
ADEELS |
5:05.32 |
5:13.64 |
8.32 |
| 3 |
Gray, Alayna G (13)
|
ADUN |
5:31.14 |
5:15.27 |
-15.87 |
| 4 |
Giumarra, Natalie A (14)
|
ADGLFY |
5:05.34 |
5:16.77 |
11.43 |
| 5 |
Leung, Adelaide Y (13)
|
ADSCHE |
5:03.86 |
5:17.89 |
14.03 |
| 6 |
Trickett, Stella M (13)
|
ADDELM |
5:33.02 |
5:19.22 |
-13.80 |
| 7 |
Mangini, Maria J (15)
|
ADSCHE |
5:09.98 |
5:25.78 |
15.80 |
| 8 |
Braun, Hannah E (16)
|
ADNTS |
5:38.76 |
5:29.00 |
-9.76 |
| 9 |
O'Donnell, Abigael F (13)
|
ADEELS |
5:39.05 |
5:40.21 |
1.16 |
| 10 |
Atanasova, Elaine P (15)
|
ADEELS |
5:19.79 |
5:47.80 |
28.01 |
| 11 |
Jensen, Jordan G (14)
|
ADUN |
5:38.78 |
5:55.99 |
17.21 |
| 12 |
McLaughlin, Brenna C (13)
|
ADSCHE |
|
6:07.56 |
- |
| 13 |
Felton, Kristin M (15)
|
ADGLFY |
|
6:16.44 |
- |
|
Decker, Elizabeth G (13)
|
ADSCHE |
|
DQ |
- |