| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hart, Jenna K (10)
|
ADSCHE |
2:21.92 |
2:17.84 |
-4.08 |
| 2 |
Fronheiser, Hannah C (12)
|
ADDELM |
2:23.37 |
2:28.83 |
5.46 |
| 3 |
Braun, Gretchen L (9)
|
ADNTS |
2:34.41 |
2:34.04 |
-0.37 |
| 4 |
Layer, Alessandra G (10)
|
ADSCHE |
2:45.17 |
2:46.84 |
1.67 |
| 5 |
Tennyson, Valerie L (10)
|
ADGLFY |
3:28.76 |
3:05.44 |
-23.32 |
| 6 |
Mienaltowski, Allison E (10)
|
ADEELS |
3:14.67 |
3:07.13 |
-7.54 |
| 7 |
O'Donnell, Maura E (9)
|
ADEELS |
2:59.68 |
3:08.06 |
8.38 |
| 8 |
Armstrong, Chloe V (10)
|
ADEELS |
3:15.50 |
3:16.76 |
1.26 |
| 9 |
Choma, Isabel (11)
|
ADUN |
|
3:30.76 |
- |
| 10 |
Sellnow, Emma M (9)
|
ADEELS |
|
3:31.42 |
- |
| 11 |
Johnston, Maya B (10)
|
ADGLFY |
3:57.59 |
3:35.90 |
-21.69 |
| 12 |
Schager, Kierra G (11)
|
ADEELS |
3:29.87 |
3:43.71 |
13.84 |