| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rivera, Alex K (16)
|
ILSPY |
28.22 |
26.32 |
-1.90 |
| 2 |
Rienstra, Nicholas M (14)
|
ILHEAT |
28.51 |
27.46 |
-1.05 |
| 3 |
Gilmore, Nicholas H (11)
|
ILSPY |
32.21 |
31.86 |
-0.35 |
| 4 |
Medlin, Charles (11)
|
ILDANY |
33.86 |
33.32 |
-0.54 |
| 5 |
Evans, Jack W (10)
|
ILSPY |
34.66 |
35.01 |
0.35 |
| 6 |
Warren, Jackson (11)
|
KNCY |
35.49 |
35.86 |
0.37 |
| 7 |
Shelton, Isaiah (11)
|
WCY |
35.03 |
37.43 |
2.40 |
| 8 |
Alleman, Landon H (9)
|
ILHEAT |
41.23 |
40.16 |
-1.07 |
| 9 |
Gray, Carter (9)
|
ILCLY |
42.99 |
40.82 |
-2.17 |
| 10 |
Tolsdorf, Jacob T (9)
|
ILSPY |
41.78 |
41.23 |
-0.55 |
| 11 |
Freeburg, Jonathan C (10)
|
ILHEAT |
40.61 |
42.34 |
1.73 |
| 12 |
Reynolds, James A (9)
|
ILHEAT |
54.40 |
54.03 |
-0.37 |
| 13 |
McMinn, Corban T (10)
|
ILHEAT |
1:02.82 |
1:02.66 |
-0.16 |