| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lai, Rachel (12)
|
ADGUY |
37.86 |
38.46 |
0.60 |
| 2 |
Mackey, Madison (12)
|
ADGUY |
38.42 |
39.00 |
0.58 |
| 3 |
Ryan, Katrina A (11)
|
ADSSCY |
40.35 |
39.41 |
-0.94 |
| 4 |
Tandlmayer, Elizabeth A (11)
|
ADSSCY |
41.04 |
39.64 |
-1.40 |
| 5 |
Sommo, Brooke M (12)
|
ADGUY |
40.93 |
39.85 |
-1.08 |
| 6 |
Benvenuto, Mariana V (12)
|
ADGLOY |
|
40.93 |
- |
| 7 |
Erb, Elisabeth J (12)
|
ADSARY |
40.91 |
41.83 |
0.92 |
| 8 |
Seleen, Claire J (11)
|
ADGLFY |
42.23 |
45.08 |
2.85 |
| 9 |
Nolan, Celia H (12)
|
ADSARY |
47.19 |
45.20 |
-1.99 |
| 10 |
Bard, Alexandria S (11)
|
ADDACC |
44.54 |
45.23 |
0.69 |
| 11 |
Teague, Lillian (11)
|
ADGUY |
45.48 |
45.33 |
-0.15 |
| 12 |
Van Woert, Maura M (11)
|
ADGUY |
45.04 |
45.54 |
0.50 |
| 13 |
Ormsby, Mikayla (11)
|
ADGGFY |
49.17 |
47.57 |
-1.60 |
| 14 |
Mitchell, Maris C (11)
|
ADDACC |
48.61 |
48.52 |
-0.09 |
| 15 |
Nicastro, Viviana A (11)
|
ADSARY |
48.34 |
50.41 |
2.07 |
| 16 |
Seltzer, Abigail J (11)
|
ADGLOY |
46.60 |
50.76 |
4.16 |
| 17 |
Johnson, Kristen L (12)
|
ADDACC |
|
54.12 |
- |
| 18 |
Schager, Kierra G (11)
|
ADDACC |
51.71 |
55.70 |
3.99 |
|
Papielion, Madison G (12)
|
ADDACC |
1:00.05 |
DQ |
- |
|
Coye, Isabella N (11)
|
ADSARY |
53.68 |
NS |
- |
|
Meng, Amy (12)
|
ADGUY |
|
NS |
- |
|
Chee, Amelia (11)
|
ADSSCY |
44.36 |
NS |
- |