| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Russel, Eden (8)
|
MABCY |
36.53 |
34.52 |
-2.01 |
| 2 |
Anderson, Xaviah (7)
|
MABCY |
37.00 |
35.45 |
-1.55 |
| 3 |
Persch, Chloe (8)
|
MABCY |
36.82 |
35.88 |
-0.94 |
| 4 |
Hunt, Mara C (8)
|
MALYM |
39.63 |
40.00 |
0.37 |
| 5 |
Bakey, Bracelyn M (8)
|
MARKRY |
38.32 |
40.77 |
2.45 |
| 6 |
Shuda, Francesca R (8)
|
MARKRY |
42.97 |
41.37 |
-1.60 |
| 7 |
O'Hara, Molly A (8)
|
MALYM |
44.18 |
42.38 |
-1.80 |
| 8 |
Inners, Elizabeth (8)
|
MABCY |
46.16 |
42.70 |
-3.46 |
| 9 |
Chidester, Keegan E (8)
|
MABCY |
44.09 |
43.35 |
-0.74 |
| 10 |
Sanders, Summer E (8)
|
MARKRY |
44.64 |
44.11 |
-0.53 |
| 11 |
Perrine-Wilson, Macy S (7)
|
MALYM |
49.84 |
44.12 |
-5.72 |
| 12 |
Hui, Emma N (7)
|
MAUMLY |
47.56 |
44.24 |
-3.32 |
| 13 |
DiFerdinando, Mai L (8)
|
MARKRY |
41.47 |
44.30 |
2.83 |
| 14 |
Banmeke, Yeside N (7)
|
MALYM |
|
44.84 |
- |
| 15 |
Silva-Trego, Mary Jane (8)
|
MABCY |
|
46.02 |
- |
| 16 |
Heckman, Molly A (8)
|
MALYM |
48.56 |
46.07 |
-2.49 |
| 17 |
Wines, Claire M (8)
|
MARKRY |
49.44 |
46.96 |
-2.48 |
| 18 |
Garg, Apeksha (8)
|
MAWGY |
48.46 |
48.56 |
0.10 |
| 19 |
Alpizar, Jazmine (8)
|
MABCY |
49.59 |
48.63 |
-0.96 |
| 20 |
Burke-Huyette, Jillian (7)
|
MARKRY |
51.59 |
48.88 |
-2.71 |
| 21 |
Radhakrishnan, Ruhi A (8)
|
MAUMLY |
49.64 |
49.45 |
-0.19 |
| 22 |
Collings, Finola M (8)
|
MARKRY |
52.35 |
49.67 |
-2.68 |
| 23 |
Burke, Abigail (8)
|
MABCY |
|
51.14 |
- |
| 24 |
Tewari, Zoya (8)
|
MALYM |
52.28 |
51.47 |
-0.81 |
| 25 |
Arun, Riona M (8)
|
MABCY |
|
55.31 |
- |
| 26 |
Senbel, Hiba (7)
|
MAWGY |
1:01.89 |
55.95 |
-5.94 |
| 27 |
Wannemacher, Olivia M (8)
|
MARKRY |
|
57.42 |
- |
| 28 |
Withers, Amelia J (8)
|
MAWGY |
1:07.41 |
58.57 |
-8.84 |