| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Nitti, Marissa R (10)
|
NIGRSC |
42.28 |
39.77 |
-2.51 |
| 2 |
Duran, Heidi K (9)
|
NIGRSC |
42.25 |
40.34 |
-1.91 |
| 3 |
Mastrosimone, Nina A (9)
|
NIWEBS |
44.41 |
44.28 |
-0.13 |
| 4 |
Brodt, Hannah C (10)
|
NIWEBS |
45.22 |
44.77 |
-0.45 |
| 5 |
Baum, Scarlett A (9)
|
NIGRSC |
45.42 |
44.82 |
-0.60 |
| 6 |
Bub, Emily R (10)
|
NIGRSC |
45.92 |
45.62 |
-0.30 |
| 7 |
Cady, Sabrina R (10)
|
NIWEBS |
45.27 |
46.46 |
1.19 |
| 8 |
Ignizio, Natalie V (10)
|
NIIRON |
46.61 |
46.57 |
-0.04 |
| 9 |
Boyum, Paige L (9)
|
NIFAST |
48.19 |
47.79 |
-0.40 |
| 10 |
Glorioso, Morgan M (10)
|
NIFAST |
48.19 |
48.25 |
0.06 |
| 11 |
Marsh, Emma L (10)
|
NIGRSC |
45.19 |
50.07 |
4.88 |
| 12 |
Aintablian, Lillyanne R (9)
|
NIIRON |
|
50.41 |
- |
| 13 |
Balkman, Zuri A (9)
|
NIGRSC |
52.00 |
52.35 |
0.35 |
| 14 |
Gouveia, Clementine A (9)
|
NIGRSC |
|
1:24.13 |
- |
|
Considine, Elizabeth A (9)
|
NIWEBS |
48.94 |
NS |
- |