| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Xu, Ming Na (10)
|
NIFAST |
31.45 |
30.30 |
-1.15 |
| 2 |
Bryce, Julia M (12)
|
NIRRST |
31.65 |
31.58 |
-0.07 |
| 3 |
Obuhanych, Zachary J (10)
|
NIUN-F |
33.90 |
34.79 |
0.89 |
| 4 |
Miller, Tyler V (12)
|
NIFAST |
37.05 |
35.98 |
-1.07 |
| 5 |
Shaw, Kathryn E (12)
|
NIWEBS |
47.61 |
36.69 |
-10.92 |
| 6 |
Visconte, Alexandria L (12)
|
NIFAST |
41.00 |
40.57 |
-0.43 |
| 7 |
Hinds, Benjamin W (8)
|
NIWEBS |
57.89 |
45.14 |
-12.75 |
| 8 |
Magnera, Sofia A (10)
|
NIWEBS |
53.30 |
45.33 |
-7.97 |
| 9 |
Resch, Rebecka J (9)
|
NIGRSC |
54.39 |
47.94 |
-6.45 |
| 10 |
Warren, Wesley C (9)
|
NIWEBS |
56.25 |
48.69 |
-7.56 |
| 11 |
Larzelere, Natalie E (9)
|
NIFAST |
|
48.98 |
- |
| 12 |
Miller, Avery K (10)
|
NIFAST |
57.05 |
49.39 |
-7.66 |
| 13 |
Siciliano, Paige E (10)
|
NIGRSC |
55.05 |
51.09 |
-3.96 |
| 14 |
Sherman, Ani E (10)
|
NIFAST |
1:02.01 |
54.75 |
-7.26 |
| 15 |
Bedford, Marin B (9)
|
NIWEBS |
|
55.54 |
- |
| 16 |
Kania, Matthew H (9)
|
NIGRSC |
57.06 |
57.67 |
0.61 |
|
Evans, Ethan D (9)
|
NIGRSC |
1:02.58 |
DQ |
- |
|
Villani, David (11)
|
NIRRST |
|
NS |
- |
|
Lorenzo, Lydia M (12)
|
NIRRST |
58.16 |
NS |
- |