| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sangasy, Khany K (11)
|
NISEAS |
36.90 |
34.72 |
-2.18 |
| 2 |
Phegley, Victoria L (11)
|
NIVICT |
35.30 |
36.21 |
0.91 |
| 3 |
Langan, Abigayle R (11)
|
NIVICT |
40.42 |
38.25 |
-2.17 |
| 4 |
Prezyna, Zoey (9)
|
NIVICT |
41.00 |
39.89 |
-1.11 |
| 5 |
Cywinski, Anna C (8)
|
NIVICT |
40.31 |
41.77 |
1.46 |
| 6 |
Kazmark, Olivia N (10)
|
NIVICT |
47.33 |
44.62 |
-2.71 |
| 7 |
Kuhlkin, Elizabeth C (10)
|
NISEAS |
51.01 |
44.84 |
-6.17 |
| 8 |
Dwivedi, Arjun M (11)
|
NISEAS |
53.34 |
52.07 |
-1.27 |
| 9 |
Nie, Amy (10)
|
NISEAS |
|
52.90 |
- |
| 10 |
Stahl, Reed P (9)
|
NIVICT |
57.47 |
57.46 |
-0.01 |
| 11 |
Noble, Mia J (9)
|
NISEAS |
1:14.36 |
59.24 |
-15.12 |
| 12 |
Ray, Gavin M (9)
|
NIVICT |
54.00 |
1:03.57 |
9.57 |
| 13 |
Cottone, Cameron A (9)
|
NIGCOM |
1:01.59 |
1:07.77 |
6.18 |
|
Pogroszewski, Frances S (9)
|
NISEAS |
58.29 |
DQ |
- |
|
Werner, Riley K (11)
|
NIVICT |
1:06.21 |
NS |
- |