| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Senglaub, Emma (14)
|
NIVICT |
33.59 |
29.64 |
-3.95 |
| 2 |
El Ghissassi, Ritaj (13)
|
NIVEST |
29.82 |
30.02 |
0.20 |
| 3 |
DeHollander, Erin K (13)
|
NIVICT |
29.99 |
30.03 |
0.04 |
| 4 |
Pathammavong, Suriyathai A (13)
|
NIVICT |
|
30.86 |
- |
| 5 |
Iosub, Lorena M (14)
|
NIVEST |
30.60 |
31.21 |
0.61 |
| 6 |
Wagner, Morgan E (10)
|
NIVICT |
36.03 |
33.14 |
-2.89 |
| 7 |
Sands, Samantha B (11)
|
ADCPP |
37.25 |
33.22 |
-4.03 |
| 8 |
Gayle, Isabella C (12)
|
ADCPP |
31.70 |
33.26 |
1.56 |
| 9 |
Wethey, Morgane A (11)
|
NIVICT |
35.27 |
34.78 |
-0.49 |
| 10 |
Kazmark, Olivia N (12)
|
NIVICT |
36.99 |
34.93 |
-2.06 |
| 11 |
Gao, Sophia (9)
|
ADCPP |
42.66 |
35.11 |
-7.55 |
| 12 |
Grigg, Ainsley (14)
|
NIKVAC |
|
35.98 |
- |
| 13 |
Callaghan, Kelsey J (13)
|
NIVEST |
36.10 |
36.12 |
0.02 |
| 14 |
Onze, Emily S (11)
|
NIVICT |
36.69 |
36.30 |
-0.39 |
| 15 |
Cywinski, Anna C (10)
|
NIVICT |
38.64 |
36.49 |
-2.15 |
| 16 |
Dickens, Jennifer E (12)
|
NIGVST |
|
36.61 |
- |
| 17 |
Hao, Helen Z (10)
|
ADCPP |
47.39 |
37.13 |
-10.26 |
| 18 |
Onze, Lauren W (11)
|
NIVICT |
37.03 |
37.25 |
0.22 |
| 19 |
Tripp, Sophia G (12)
|
NIVICT |
|
40.09 |
- |
| 20 |
Miller, Amanda C (10)
|
NIVICT |
|
41.76 |
- |
| 21 |
Vallone, Francesca G (10)
|
NIVEST |
45.07 |
45.99 |
0.92 |