| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kooyers, Taryn K (15)
|
GUSWIM |
25.25 |
25.56 |
0.31 |
| 2 |
Kriendler, Lauren E (16)
|
GUSWIM |
25.73 |
26.29 |
0.56 |
| 3 |
Johnson, Riley S (15)
|
GUSWIM |
26.29 |
27.19 |
0.90 |
| 4 |
McGillis, Joni J (15)
|
GUTEST |
27.38 |
27.72 |
0.34 |
| 5 |
Reeves, Emma M (16)
|
GUSCAT |
27.09 |
28.05 |
0.96 |
| 6 |
Gibson, Emily E (15)
|
GUTEST |
28.48 |
28.33 |
-0.15 |
| 7 |
Kapoor, Shivani (17)
|
GUTEST |
27.31 |
28.72 |
1.41 |
| 8 |
Hoffman, Mackenzie L (16)
|
GUSWIM |
28.88 |
28.78 |
-0.10 |
| 9 |
Brosch, Kaylee G (15)
|
GUPFL |
|
28.79 |
- |
| 10 |
Perez, Gina R (15)
|
GUPFL |
|
28.97 |
- |
| 11 |
Zwerneman, Hannah N (18)
|
GUTTST |
27.97 |
29.64 |
1.67 |
| 12 |
Freeman, Madison A (16)
|
GUTYES |
|
29.70 |
- |
| 13 |
Musselman, Madison P (15)
|
GUKATY |
29.14 |
29.74 |
0.60 |
| 14 |
Findley, Lauren N (15)
|
GUVSST |
|
29.81 |
- |
| 15 |
Peden, Emily G (15)
|
GUKATY |
30.63 |
31.41 |
0.78 |
|
Osorio, Britney W (16)
|
GUSWIM |
33.44 |
NS |
- |
|
Wilson, Tatum K (16)
|
GULIFE |
|
NS |
- |
|
Aly, Mia Y (15)
|
GULIFE |
33.13 |
NS |
- |
|
Watson, Linsey M (15)
|
GUKATY |
26.82 |
NS |
- |