| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Sosa, Lylah E (9)
|
GUUN-B |
|
2:56.11 |
- |
| 2 |
Zhao, Serena (9)
|
GU300 |
3:34.06 |
2:56.83 |
-37.23 |
| 3 |
Chen, Gwyneth E (9)
|
GUEPRC |
3:03.68 |
2:57.65 |
-6.03 |
| 4 |
Qiu, Annabel Y (10)
|
GU300 |
3:06.68 |
2:59.30 |
-7.38 |
| 5 |
Hornes, Georgia P (10)
|
GUTASC |
3:21.54 |
3:07.18 |
-14.36 |
| 6 |
Coreas, Naileah C (10)
|
GUUSA |
|
3:14.14 |
- |
| 7 |
Liu, Amelia (10)
|
GU300 |
|
3:18.12 |
- |
| 8 |
Fields, Evelyn E (9)
|
GUFRST |
|
3:22.52 |
- |
| 9 |
Hynes, Ansley P (9)
|
GUVSST |
3:25.47 |
3:23.30 |
-2.17 |
| 10 |
Porter, Makenna L (10)
|
GUBATS |
|
3:27.66 |
- |
| 11 |
Nguyen, Alanna H (10)
|
GULSST |
|
3:38.37 |
- |
| 12 |
Overman, Elaine I (9)
|
GUTASC |
|
3:42.51 |
- |
| 13 |
Hartfiel, Madeline G (9)
|
GUFRST |
|
3:42.66 |
- |
| 14 |
Delafosse, Ava R (9)
|
GUFRST |
|
3:42.97 |
- |
| 15 |
Fehlis, Emma G (10)
|
GUTASC |
3:55.63 |
3:45.78 |
-9.85 |
| 16 |
Espitia, Sofia (9)
|
GUVSST |
|
3:49.22 |
- |
| 17 |
Gill, Caroline R (10)
|
GUTASC |
|
3:51.55 |
- |
|
Montgomery, Lillian D (9)
|
GULSST |
|
DQ |
- |
|
Melton, Riley E (10)
|
GUTASC |
|
DQ |
- |
|
Denner, Jaylyn R (10)
|
GUFRST |
|
DQ |
- |