| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeSantis, Justin G (6)
|
MDCGA |
24.91 |
22.90 |
-2.01 |
| 2 |
Li, Weiming A (6)
|
MDCAA |
30.92 |
25.99 |
-4.93 |
| 3 |
Chew, William (6)
|
MDRAC |
29.46 |
26.04 |
-3.42 |
| 4 |
DeVault, Daniel R (6)
|
MDNBAC |
27.50 |
27.12 |
-0.38 |
| 5 |
Rutz, Griffin B (6)
|
MDYCM |
27.30 |
27.25 |
-0.05 |
| 6 |
Yetso, Olsen G (6)
|
MDYCM |
|
29.30 |
- |
| 7 |
Ballentine, Troy A (5)
|
MDFAST |
|
29.68 |
- |
| 8 |
Gilliland, Daniel P (6)
|
MDSPRC |
28.20 |
30.13 |
1.93 |
| 9 |
Torres-Naranjo, Samuel (6)
|
MDCGA |
32.46 |
31.53 |
-0.93 |
|
Furgason, Roran J (6)
|
MDACA |
23.95 |
NS |
- |
|
Borisov, Mark O (6)
|
MDFAST |
|
NS |
- |
|
Muniz-Berrios, Ryan J (6)
|
MDFAST |
46.80 |
NS |
- |
|
Cunningham, Liam B (6)
|
MDSPRC |
24.76 |
NS |
- |
|
Cleghorn, Grayson S (5)
|
MDYCM |
|
NS |
- |
|
Quinn, Riley (6)
|
MDYCM |
27.63 |
NS |
- |