| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Scherer, Ellie D (16)
|
MDLBA |
2:28.26 |
2:25.20 |
-3.06 |
| 2 |
Helminiak, Katherine E (15)
|
MDMAS |
2:27.13 |
2:29.08 |
1.95 |
| 3 |
Thorogood, Tanith A (15)
|
MDCAA |
2:32.96 |
2:29.19 |
-3.77 |
| 4 |
Patz, Sophia N (16)
|
MDSPRC |
2:30.08 |
2:30.09 |
0.01 |
| 5 |
Byers, Hope A (15)
|
MDLBA |
2:26.43 |
2:35.09 |
8.66 |
| 6 |
Lundquist, Ainsley S (17)
|
MDMAS |
2:38.21 |
2:39.21 |
1.00 |
|
Rollins, Anna N (16)
|
MDCAA |
2:32.32 |
NS |
- |
|
Cattaneo, Isabella M (16)
|
MDLBA |
2:27.07 |
NS |
- |
|
Hewitt, Kenley G (16)
|
MDLBA |
2:29.23 |
NS |
- |
|
Fields, Kelly A (16)
|
MDMAS |
2:25.51 |
NS |
- |
|
Laughlin, Sydney I (18)
|
MDMAS |
2:26.80 |
NS |
- |
|
Parker, Mackenzie A (15)
|
MDSPRC |
3:01.76 |
NS |
- |