| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wong, Victoria (12)
|
NJSCAR |
38.14 |
44.42 |
6.28 |
| 2 |
Gaston, Gabriella M (12)
|
NJLHY |
38.54 |
44.52 |
5.98 |
| 3 |
McNamara, Charlotte R (11)
|
NJLHY |
39.23 |
44.70 |
5.47 |
| 4 |
Davies, Zoe E (11)
|
NJLHY |
37.32 |
44.85 |
7.53 |
| 5 |
Keys, Halle A (12)
|
NJOCY |
37.99 |
45.24 |
7.25 |
| 6 |
Kim, Heesoo (11)
|
NJSVY |
39.20 |
45.61 |
6.41 |
| 7 |
Dvorkin, Dora R (12)
|
NJSTAC |
40.75 |
45.79 |
5.04 |
| 8 |
Blomquist, Lila M (12)
|
NJSTAC |
38.75 |
46.56 |
7.81 |
| 9 |
Chen, Sunny L (11)
|
NJSVY |
41.26 |
46.73 |
5.47 |
| 10 |
Vargas, Kaylee A (12)
|
NJSTAC |
41.20 |
49.34 |
8.14 |
| 11 |
Cohn, Ella M (11)
|
NJOCY |
50.57 |
49.86 |
-0.71 |
| 12 |
Demand, Ella G (12)
|
NJOCY |
41.44 |
49.99 |
8.55 |
| 13 |
Newsome, Nicolette S (12)
|
NJSTAC |
41.80 |
50.49 |
8.69 |
| 14 |
Yao, Gloria (11)
|
NJSVY |
41.74 |
50.70 |
8.96 |
| 15 |
Luo, JingJing J (11)
|
NJSVY |
49.02 |
56.84 |
7.82 |