| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cao, Cecilia Y (13)
|
ADDELM |
35.22 |
40.54 |
5.32 |
| 2 |
Bearor, Anna L (14)
|
ADGLFY |
41.98 |
43.06 |
1.08 |
| 3 |
Tennyson, Valerie L (11)
|
ADGLFY |
44.96 |
44.68 |
-0.28 |
| 4 |
Goodspeed, Fayth R (10)
|
ADGLEN |
|
45.96 |
- |
| 5 |
Mienaltowski, Allison E (12)
|
ADEELS |
50.88 |
47.11 |
-3.77 |
| 6 |
Gilbert, Kendall G (12)
|
ADGLEN |
48.81 |
48.59 |
-0.22 |
| 7 |
Bard, Alexandria S (12)
|
ADEELS |
|
49.10 |
- |
| 8 |
Kaufman, Ava R (11)
|
ADDELM |
|
49.51 |
- |
| 9 |
Mohamed, Amber H (11)
|
ADSSCY |
48.02 |
50.46 |
2.44 |
| 10 |
Mitchell, Maris C (12)
|
ADEELS |
|
50.47 |
- |
| 11 |
Fitzgerald, Catherine G (12)
|
ADDELM |
44.35 |
50.68 |
6.33 |
| 12 |
Sellnow, Abigail V (12)
|
ADEELS |
52.57 |
51.41 |
-1.16 |
| 13 |
Bonesteel, Jana M (11)
|
ADGLFY |
|
52.23 |
- |
| 14 |
Bearor, McKenna M (11)
|
ADGLFY |
55.44 |
52.86 |
-2.58 |
| 15 |
Fiske, Giana M (11)
|
ADGLFY |
|
53.75 |
- |
| 16 |
Sellnow, Emma M (10)
|
ADEELS |
54.61 |
54.66 |
0.05 |
| 17 |
Ziter, Sophie L (9)
|
ADSSCY |
56.33 |
57.32 |
0.99 |
| 18 |
Carter, Gwendolyn S (10)
|
ADEELS |
|
57.53 |
- |
| 19 |
Yafayeva, Roxana Z (10)
|
ADDELM |
45.96 |
58.38 |
12.42 |
| 21 |
Huang, Rylie Z (8)
|
ADSSCY |
1:04.46 |
1:06.11 |
1.65 |
| 22 |
Kropp, Paige H (10)
|
ADEELS |
|
1:10.20 |
- |
|
O'Donnell, Bridgett M (7)
|
ADEELS |
|
DQ |
- |
|
Krenav, Anna E (7)
|
ADEELS |
|
DQ |
- |
|
Durante, Skylar I (12)
|
ADGLFY |
|
NS |
- |
|
Jerabek, Ila R (11)
|
ADDELM |
45.24 |
NS |
- |
|
Heckler, Felicity A (11)
|
ADDELM |
53.29 |
NS |
- |