| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bearor, Anna L (14)
|
ADGLFY |
36.86 |
37.29 |
0.43 |
| 2 |
Hopper, Olivia K (12)
|
ADDELM |
31.36 |
37.80 |
6.44 |
| 3 |
Tennyson, Valerie L (11)
|
ADGLFY |
43.26 |
39.17 |
-4.09 |
| 4 |
Frazee, Virginia V (14)
|
ADDELM |
31.97 |
39.29 |
7.32 |
| 5 |
Sellnow, Abigail V (12)
|
ADEELS |
39.19 |
40.74 |
1.55 |
| 6 |
Bard, Alexandria S (12)
|
ADEELS |
|
40.92 |
- |
| 7 |
Fink, Jayme S (13)
|
ADDELM |
40.97 |
41.76 |
0.79 |
| 8 |
Mitchell, Maris C (12)
|
ADEELS |
|
42.93 |
- |
| 9 |
Swan-Scott, Rowan M (12)
|
ADGLFY |
35.38 |
43.75 |
8.37 |
| 10 |
Bearor, McKenna M (11)
|
ADGLFY |
42.99 |
44.13 |
1.14 |
| 11 |
Gilbert, Kendall G (12)
|
ADGLEN |
44.01 |
44.40 |
0.39 |
| 12 |
Mienaltowski, Allison E (12)
|
ADEELS |
46.07 |
44.73 |
-1.34 |
| 13 |
Bonesteel, Jana M (11)
|
ADGLFY |
51.74 |
45.75 |
-5.99 |
| 14 |
Huang, Madison Z (11)
|
ADSSCY |
40.64 |
45.93 |
5.29 |
| 15 |
Kaufman, Ava R (11)
|
ADDELM |
|
47.28 |
- |
| 16 |
Yafayeva, Roxana Z (10)
|
ADDELM |
38.49 |
50.61 |
12.12 |
| 17 |
Huang, Rylie Z (8)
|
ADSSCY |
51.06 |
52.67 |
1.61 |
| 18 |
Kropp, Paige H (10)
|
ADEELS |
|
52.71 |
- |
| 19 |
Sellnow, Emma M (10)
|
ADEELS |
51.97 |
53.36 |
1.39 |
| 20 |
Carter, Gwendolyn S (10)
|
ADEELS |
1:03.89 |
55.38 |
-8.51 |
| 21 |
Krenav, Anna E (7)
|
ADEELS |
|
1:03.12 |
- |
| 22 |
Conroy, Isabella S (7)
|
ADSIDN |
|
1:03.57 |
- |
| 23 |
O'Donnell, Bridgett M (7)
|
ADEELS |
|
1:18.66 |
- |
|
Goodspeed, Fayth R (10)
|
ADGLEN |
|
NS |
- |