| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Rose, Sydney S (12)
|
NCCVAC |
33.82 |
33.87 |
0.05 |
| 2 |
Thomas, Paige D (12)
|
NCGG |
35.70 |
35.35 |
-0.35 |
| 3 |
Fox, Emma J (12)
|
NCCVAC |
37.83 |
36.04 |
-1.79 |
| 4 |
Sacchetti, Isabella M (12)
|
NCMSC |
34.77 |
36.84 |
2.07 |
| 5 |
Clontz, Leah J (12)
|
NCCVAC |
40.18 |
38.68 |
-1.50 |
| 6 |
Outlaw, Lizzie L (12)
|
NCWA |
43.97 |
39.97 |
-4.00 |
| 7 |
Beltran-Trejo, Nicole (11)
|
NCCVAC |
51.21 |
41.21 |
-10.00 |
| 8 |
Aragon-Zamora, Angelica (12)
|
NCCVAC |
46.78 |
43.85 |
-2.93 |
| 9 |
Rautiola, Larissa L (12)
|
NCCVAC |
39.24 |
44.36 |
5.12 |
| 10 |
Lopez-Rodriquez, Naydelin D (11)
|
NCMSC |
47.45 |
46.23 |
-1.22 |
| 11 |
Huffstickler, Anna E (12)
|
NCGG |
45.34 |
46.38 |
1.04 |
| 12 |
Carswell, Kennedy G (12)
|
NCMSC |
50.75 |
50.04 |
-0.71 |
|
Lindquist, Kathryn M (11)
|
NCGG |
42.71 |
NS |
- |