| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Casey, Garrett P (12)
|
NISWAT |
31.25 |
30.92 |
-0.33 |
| 2 |
Dunn, Matthew E (11)
|
NIHHSC |
30.25 |
32.34 |
2.09 |
| 3 |
Comstock, Samuel F (11)
|
NIHHSC |
32.56 |
32.42 |
-0.14 |
| 4 |
Weseman, Gavin L (12)
|
NIEMAC |
33.70 |
32.52 |
-1.18 |
| 5 |
Merrill, Christian T (12)
|
NIGWST |
34.45 |
34.86 |
0.41 |
| 6 |
Jones, William P (11)
|
NIPVSC |
36.10 |
34.97 |
-1.13 |
| 7 |
Brophy, Elijah M (12)
|
NIGVST |
36.60 |
36.01 |
-0.59 |
| 8 |
Clinch, Jonathan T (11)
|
NIAAAC |
38.47 |
37.10 |
-1.37 |
| 9 |
Hammer, Tristan J (11)
|
NISWAT |
36.90 |
39.20 |
2.30 |
| 10 |
Abram-Hopkins, Wesley A (11)
|
NIAAAC |
38.00 |
40.69 |
2.69 |
| 11 |
Polcz, Jesse N (11)
|
NIAAAC |
37.79 |
41.39 |
3.60 |
| 12 |
Peterson-Volz, Lucas S (11)
|
NIEMAC |
42.83 |
43.18 |
0.35 |