| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Greene, Emily M (12)
|
NIAAAC |
29.45 |
29.49 |
0.04 |
| 2 |
Sauer, Emma L (11)
|
NIHAMB |
31.03 |
31.00 |
-0.03 |
| 3 |
Zawodzinski, Zoe E (11)
|
NIHAMB |
30.05 |
31.27 |
1.22 |
| 4 |
Bement, Kelly D (12)
|
NIHAMB |
32.32 |
32.61 |
0.29 |
| 5 |
Kless, Ellinor L (12)
|
NIAAAC |
34.61 |
35.11 |
0.50 |
| 6 |
Miller, Ella R (11)
|
NIHAMB |
35.69 |
35.21 |
-0.48 |
| 7 |
Uhteg, Julia D (11)
|
NISWAT |
36.21 |
36.45 |
0.24 |
| 8 |
Mancuso, Gabriella J (11)
|
NIHAMB |
39.00 |
37.94 |
-1.06 |
| 9 |
Romance, Payton E (12)
|
NIAAAC |
40.77 |
41.28 |
0.51 |
| 10 |
LaVerdi, Emma K (12)
|
NIHAMB |
1:28.31 |
41.86 |
-46.45 |
| 11 |
Evans, Natalie P (11)
|
NIHAMB |
44.31 |
42.79 |
-1.52 |
| 12 |
Bagley, Cassandra C (11)
|
NISWAT |
54.36 |
44.60 |
-9.76 |
| 13 |
Schmitt, Larrissa R (11)
|
NIHAMB |
49.30 |
46.33 |
-2.97 |
|
George, Samantha L (12)
|
NIAAAC |
30.33 |
NS |
- |
|
Marcheson, Madison E (12)
|
NIAAAC |
34.85 |
NS |
- |