| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marshall, Molly E (10)
|
NIEMAC |
47.46 |
38.29 |
-9.17 |
| 2 |
Christopher, Hailey R (10)
|
NIGWST |
37.94 |
38.50 |
0.56 |
| 3 |
Herman, Tyyetta R (10)
|
NIFKVL |
40.64 |
38.68 |
-1.96 |
| 4 |
Gehm, Emmalie R (9)
|
NIEMAC |
38.30 |
38.70 |
0.40 |
| 5 |
Price, Elizabeth E (9)
|
NIFKVL |
45.36 |
40.12 |
-5.24 |
| 6 |
Whitepigeon, Breana M (10)
|
NIGWST |
45.23 |
44.95 |
-0.28 |
| 7 |
Chase, Abbigale B (9)
|
NIFKVL |
1:22.93 |
46.63 |
-36.30 |
| 8 |
Budinger, Emily A (10)
|
NIGVST |
51.46 |
46.84 |
-4.62 |
| 9 |
Mitchell, Alexandra J (9)
|
NIEMAC |
48.48 |
48.02 |
-0.46 |
| 10 |
McAuley, Catherine G (10)
|
NIFKVL |
55.13 |
48.30 |
-6.83 |
| 11 |
Kerr, Bell L (9)
|
NIPVSC |
44.51 |
48.41 |
3.90 |
| 12 |
Boussa, Serena M (10)
|
NIGVST |
1:17.16 |
56.45 |
-20.71 |
| 13 |
Hillman, Ana G (9)
|
NIFKVL |
47.40 |
59.76 |
12.36 |
| 14 |
Kruszka, Sarah R (9)
|
NIPVSC |
|
1:05.59 |
- |
| 15 |
Yowell, Kaitlin L (9)
|
NIEMAC |
|
1:08.16 |
- |
|
Bennet, Payton A (9)
|
NIAAAC |
45.23 |
NS |
- |
|
Potter, Isabella (10)
|
NIGVST |
37.07 |
NS |
- |
|
Mentley-Peters, Willow S (10)
|
NIGWST |
52.29 |
NS |
- |
|
Hohl, Paige M (9)
|
NIPVSC |
49.56 |
NS |
- |