| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Greene, Emily M (12)
|
NIAAAC |
29.97 |
29.45 |
-0.52 |
| 2 |
Jackson, Megan L (11)
|
NIFKVL |
32.23 |
30.04 |
-2.19 |
| 3 |
Cadle, Mattison I (12)
|
NIPVSC |
30.02 |
30.44 |
0.42 |
| 4 |
George, Samantha L (12)
|
NIAAAC |
30.33 |
30.69 |
0.36 |
| 5 |
Christopher, Emma E (12)
|
NIGWST |
30.50 |
31.52 |
1.02 |
| 6 |
Slavinski, Anna C (11)
|
NIFKVL |
32.87 |
32.03 |
-0.84 |
| 7 |
Liu, Sarah Z (11)
|
NIEMAC |
34.84 |
32.14 |
-2.70 |
| 8 |
Lang, Ellianna G (12)
|
NIAAAC |
32.63 |
33.05 |
0.42 |
| 9 |
Keesler, Kaylynn N (12)
|
NIEMAC |
33.86 |
33.92 |
0.06 |
| 10 |
Dahl, Sydney R (12)
|
NIPVSC |
32.61 |
34.09 |
1.48 |
| 11 |
Kless, Ellinor L (12)
|
NIAAAC |
39.62 |
34.61 |
-5.01 |
| 12 |
Marcheson, Madison E (12)
|
NIAAAC |
37.27 |
34.85 |
-2.42 |
| 13 |
Sternisha, Madison N (12)
|
NIGWST |
35.46 |
36.95 |
1.49 |
| 14 |
Herman, Tarryn G (12)
|
NIFKVL |
39.04 |
37.67 |
-1.37 |
| 15 |
LaBenne, Ellie B (12)
|
NIGVST |
43.40 |
38.37 |
-5.03 |
| 16 |
Ainsworth, Cayleigh R (11)
|
NIFKVL |
42.62 |
39.33 |
-3.29 |
| 17 |
Merrill, Cameron R (12)
|
NIGWST |
39.31 |
39.81 |
0.50 |
| 18 |
Romance, Payton E (11)
|
NIAAAC |
40.77 |
42.83 |
2.06 |
| 19 |
Kennedy, Alexandria (11)
|
NIGWST |
|
44.16 |
- |
| 20 |
Thompson, Lily M (11)
|
NIFKVL |
46.13 |
46.36 |
0.23 |
| 21 |
Forrester, Katie L (11)
|
NIFKVL |
52.52 |
47.38 |
-5.14 |
| 22 |
Rose, Marissa K (12)
|
NIFKVL |
53.02 |
49.20 |
-3.82 |
| 23 |
Courtney, Sarah E (11)
|
NIFKVL |
54.11 |
53.87 |
-0.24 |
| 24 |
Wetherby, Breanne A (12)
|
NIEMAC |
|
59.91 |
- |
|
Zywiczynski, Gracie A (11)
|
NIAAAC |
38.83 |
NS |
- |
|
Bartlett, Aurora S (11)
|
NIGWST |
37.26 |
NS |
- |
|
WhitePigeon, Keely R (12)
|
NIGWST |
36.29 |
NS |
- |