| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burlingame, Olivia A (7)
|
NIHHSC |
|
25.51 |
- |
| 2 |
Deibler, Reagan L (8)
|
NIEMAC |
29.14 |
27.85 |
-1.29 |
| 3 |
Szumigala, Alexis R (8)
|
NIPVSC |
26.45 |
27.94 |
1.49 |
| 4 |
Brown, Ellouise S (7)
|
NISAAC |
|
31.08 |
- |
| 5 |
Sullivan, Monroe M (5)
|
NIFKVL |
31.61 |
33.56 |
1.95 |
| 6 |
Monin, Tyce S (8)
|
NIAAAC |
35.72 |
33.64 |
-2.08 |
| 7 |
Dougherty, Steven C (5)
|
NIGVST |
|
36.47 |
- |
| 8 |
Schram, Dawson P (6)
|
NIEMAC |
38.07 |
42.33 |
4.26 |
| 9 |
Ruszkowski, Alan F (7)
|
NIEMAC |
43.94 |
51.14 |
7.20 |
| 10 |
Shutt, Rowan J (6)
|
NIGVST |
|
1:00.00 |
- |
|
Slocum, Stella J (6)
|
NIAAAC |
33.49 |
DQ |
- |
|
Aftuck, Natalie G (6)
|
NIGVST |
36.54 |
DQ |
- |
|
Tronetti, Seraphina G (7)
|
NIGVST |
|
NS |
- |