| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Waldon, Hayden E (12)
|
NIGVST |
28.55 |
29.39 |
0.84 |
| 2 |
Kinnicutt, Grace A (12)
|
NIGVST |
|
31.03 |
- |
| 3 |
Gehm, Emmalie R (11)
|
NIGVST |
|
31.51 |
- |
| 4 |
Price, Elizabeth E (11)
|
NIFKVL |
31.35 |
31.55 |
0.20 |
| 5 |
DeGroff, Katrina A (12)
|
NIEMAC |
32.24 |
31.79 |
-0.45 |
| 6 |
Quigley, Camryn A (12)
|
NISAAC |
37.79 |
34.19 |
-3.60 |
| 7 |
Bidzerkowny, Alexandra E (12)
|
NIGVST |
34.31 |
35.35 |
1.04 |
| 8 |
Hand, Cassidy R (12)
|
NIGVST |
36.15 |
36.21 |
0.06 |
| 9 |
VanEtten, Megan T (12)
|
NIGVST |
40.81 |
38.30 |
-2.51 |
| 10 |
Rohwer, Kelsey L (12)
|
NISAAC |
40.93 |
42.48 |
1.55 |
| 11 |
Boussa, Serena M (12)
|
NIGVST |
40.50 |
42.85 |
2.35 |
| 12 |
Yarbour, Kaya (11)
|
NISAAC |
41.77 |
43.70 |
1.93 |
| 13 |
Maybee, Marlee M (12)
|
NISAAC |
42.86 |
43.83 |
0.97 |
| 14 |
John, Lauren E (11)
|
NISAAC |
45.22 |
45.10 |
-0.12 |
| 15 |
Garrey, Trinity K (11)
|
NIFKVL |
47.25 |
48.21 |
0.96 |
| 16 |
Jimerson, Destiney R (11)
|
NISAAC |
|
48.66 |
- |