| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Clark, Madison A (10)
|
VAPSDN |
3:31.88 |
3:12.03 |
-19.85 |
| 2 |
Toepp, Kensington J (10)
|
VANOVA |
3:18.46 |
3:17.57 |
-0.89 |
| 3 |
Mosby-Velasco, Arianna M (10)
|
VAQSTS |
3:25.20 |
3:22.53 |
-2.67 |
| 4 |
Crotts, Kylee N (10)
|
VASRVA |
3:21.05 |
3:27.50 |
6.45 |
| 5 |
Smolka, Rebecca B (10)
|
VANOVA |
|
3:37.15 |
- |
| 6 |
Harvey, Parker J (9)
|
VANOVA |
4:14.48 |
3:40.81 |
-33.67 |
| 7 |
Gantz, Kara E (9)
|
VAUN-N |
4:01.78 |
3:46.26 |
-15.52 |
| 8 |
Weingartner, Alyssa K (9)
|
VAPSDN |
|
3:47.85 |
- |
|
Patterson, Mackenzie J (9)
|
VANOVA |
|
DQ |
- |
|
Smith, Stella P (9)
|
VAPSDN |
|
DQ |
- |