| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Thomas, Alexis G (10)
|
VANOVA |
43.18 |
41.78 |
-1.40 |
| 2 |
Douglas, Isabella V (9)
|
VANOVA |
47.31 |
45.23 |
-2.08 |
| 3 |
Ezzell, Lillian J (9)
|
VANOVA |
48.98 |
45.25 |
-3.73 |
| 4 |
Gupton, Annie F (9)
|
VANOVA |
44.86 |
45.85 |
0.99 |
| 5 |
Green, Elizabeth H (10)
|
VANOVA |
47.95 |
46.33 |
-1.62 |
| 6 |
Smith, Macey E (9)
|
VANOVA |
50.24 |
46.79 |
-3.45 |
| 7 |
Tambellini, Natalie G (10)
|
VANOVA |
49.20 |
47.19 |
-2.01 |
| 8 |
Smith, Eloise L (10)
|
VANOVA |
|
48.91 |
- |
| 9 |
Mundy, Elinor C (9)
|
VANOVA |
53.30 |
50.89 |
-2.41 |
| 10 |
Rockelein, Alexandra J (9)
|
VANOVA |
1:01.35 |
51.41 |
-9.94 |
| 11 |
Bryant, Willa K (9)
|
VANOVA |
49.53 |
52.37 |
2.84 |
| 12 |
Bouchard, Isabelle A (9)
|
VANOVA |
56.29 |
52.39 |
-3.90 |
| 13 |
Farmer, Elizabeth G (9)
|
VANOVA |
57.84 |
53.15 |
-4.69 |
| 14 |
Singh, Kaaya (9)
|
VANOVA |
52.15 |
54.73 |
2.58 |
| 15 |
Beazley, Rylan (9)
|
VANOVA |
|
55.35 |
- |