| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Hollender, Brighton C (7)
|
VAQSTS |
25.11 |
24.83 |
-0.28 |
| 2 |
Libby, Katherine Z (7)
|
VANOVA |
26.75 |
25.20 |
-1.55 |
| 3 |
Raulerson, Marley T (7)
|
VANOVA |
26.94 |
25.90 |
-1.04 |
| 4 |
Sunkavalli, Chaithra (7)
|
VANOVA |
27.75 |
26.26 |
-1.49 |
| 5 |
Wirt, Caroline F (7)
|
VAPSDN |
34.12 |
26.30 |
-7.82 |
| 6 |
Browning, Scarlett E (7)
|
VANOVA |
28.23 |
26.57 |
-1.66 |
| 7 |
Melson, Meriwether G (7)
|
VANOVA |
27.59 |
27.17 |
-0.42 |
| 8 |
Handral, Dwani P (7)
|
VANOVA |
29.81 |
28.74 |
-1.07 |
| 9 |
Hromyak, Alexis (7)
|
VANOVA |
|
30.01 |
- |
| 10 |
Kulkarni, Aranyaa (7)
|
VACA-Y |
31.42 |
32.38 |
0.96 |
| 11 |
Winslow, Eliza B (7)
|
VANOVA |
32.17 |
33.07 |
0.90 |
| 12 |
Ravuru, Aadhya (7)
|
VANOVA |
34.33 |
33.40 |
-0.93 |
| 13 |
Allen, Josephine A (7)
|
VANOVA |
32.68 |
33.48 |
0.80 |
| 14 |
Gutierrez, Julianna M (7)
|
VANOVA |
33.80 |
33.88 |
0.08 |
| 15 |
Murphy, Serena C (7)
|
VANOVA |
48.60 |
34.82 |
-13.78 |
| 16 |
Perea Romero, Maricielo R (7)
|
VANOVA |
34.41 |
35.07 |
0.66 |
| 17 |
Klipp, Mackenzie K (7)
|
VAQSTS |
43.91 |
36.50 |
-7.41 |
| 18 |
Green, Stella D (7)
|
VANOVA |
40.03 |
40.75 |
0.72 |