| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Lindsey C (14)
|
VASRVA |
|
27.56 |
- |
| 2 |
Grumiaux, Taylor J (13)
|
VASRVA |
28.43 |
28.30 |
-0.13 |
| 3 |
Stevens, Eliza P (13)
|
VAUN-N |
31.05 |
29.30 |
-1.75 |
| 4 |
Popik, Elizabeth J (13)
|
VANOVA |
28.29 |
29.55 |
1.26 |
| 5 |
Maksimovic, Sara (13)
|
VANOVA |
28.32 |
29.88 |
1.56 |
| 6 |
Hollinshead, Hannah M (13)
|
VASRVA |
|
29.93 |
- |
| 7 |
Kurtz, Jessica E (13)
|
VASRVA |
|
30.01 |
- |
| 8 |
Krach, Brooklyn M (13)
|
VANOVA |
29.85 |
30.20 |
0.35 |
| 9 |
Burdsall, Veronica E (13)
|
VANOVA |
30.42 |
30.65 |
0.23 |
| 10 |
Marshall, Grace E (14)
|
VANOVA |
31.27 |
30.96 |
-0.31 |
| 11 |
McCullough, Elizabeth A (14)
|
VANOVA |
31.46 |
31.06 |
-0.40 |
| 12 |
Benhamed, Leila I (14)
|
VANOVA |
33.96 |
31.08 |
-2.88 |
| 13 |
Reilly, Rachel M (14)
|
VANOVA |
31.47 |
31.24 |
-0.23 |
| 14 |
McCullough, Allison L (14)
|
VANOVA |
31.56 |
31.65 |
0.09 |
| 15 |
Nanayakkara, Sanuthi D (13)
|
VANOVA |
35.69 |
34.32 |
-1.37 |
| 16 |
Altermatt, Jamie N (14)
|
VANOVA |
36.51 |
37.63 |
1.12 |