| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Prillaman, Caroline E (7)
|
VAPSDN |
|
40.87 |
- |
| 2 |
Derouaux, Hunter M (7)
|
VASRVA |
|
43.36 |
- |
| 3 |
Trivette, Emma B (7)
|
VANOVA |
44.04 |
45.36 |
1.32 |
| 4 |
Cousins, Madison Q (7)
|
VANOVA |
|
46.22 |
- |
| 5 |
Flynn, Amelia L (7)
|
VASRVA |
1:08.00 |
50.83 |
-17.17 |
| 6 |
Edwards, Ella M (7)
|
VANOVA |
|
56.33 |
- |
| 7 |
Vaclavik, Elizabeth P (7)
|
VANOVA |
|
57.65 |
- |
| 8 |
Broocker, Elizabeth C (7)
|
VANOVA |
|
1:05.79 |
- |
| 9 |
Zubowsky, Autumn M (7)
|
VANOVA |
|
1:06.02 |
- |
| 10 |
McDearmon, Meredith J (7)
|
VANOVA |
1:08.08 |
1:08.74 |
0.66 |
| 11 |
Lokhande, Aahana S (7)
|
VANOVA |
|
1:13.14 |
- |
| 12 |
Barna, Alaina J (7)
|
VAPSDN |
|
1:13.84 |
- |
|
Joyner, Katherine G (7)
|
VAPSDN |
50.49 |
NS |
- |