| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Estrada, Natalie (12)
|
NTDM |
44.49 |
34.14 |
-10.35 |
| 2 |
Dong, Emily H (12)
|
NTCOPS |
30.31 |
34.17 |
3.86 |
| 3 |
Dewhirst, Isabella N (12)
|
NTUNAT |
30.99 |
34.92 |
3.93 |
| 4 |
Story, Kennedy G (12)
|
NTCOPS |
44.81 |
35.74 |
-9.07 |
| 5 |
Edwards, Simone A (11)
|
NTDM |
45.79 |
37.32 |
-8.47 |
| 6 |
Beeson, Grier G (11)
|
NTUNAT |
39.68 |
37.94 |
-1.74 |
| 7 |
Obideiko, Veronika (12)
|
NTCOPS |
38.66 |
40.32 |
1.66 |
| 8 |
Chang, Chloe S (11)
|
NTDM |
53.60 |
40.35 |
-13.25 |
| 9 |
Doolittle, Charlotte P (12)
|
NTSTAR |
45.16 |
40.88 |
-4.28 |
| 10 |
Dehmer, Harper A (11)
|
NTDM |
40.20 |
40.94 |
0.74 |
| 11 |
Osman, Heba (11)
|
NTCOPS |
41.05 |
41.07 |
0.02 |
| 12 |
Spiride, Ana M (11)
|
NTCOPS |
37.95 |
42.03 |
4.08 |
| 13 |
Peck, Penelope L (12)
|
NTUNAT |
39.05 |
43.77 |
4.72 |
| 14 |
Sennett, Madison M (11)
|
NTSTAR |
41.03 |
44.07 |
3.04 |
| 15 |
Mandrell, Olivia J (11)
|
NTATAC |
37.76 |
45.12 |
7.36 |
| 16 |
Lepp, Charlotte S (11)
|
NTDM |
40.55 |
45.28 |
4.73 |
| 17 |
Lim, Heather H (11)
|
NTSTAR |
38.87 |
45.71 |
6.84 |
| 18 |
Zucker, Yael (11)
|
NTLIFE |
|
45.77 |
- |
| 19 |
Riemer, Abigail D (12)
|
NTDM |
42.75 |
46.36 |
3.61 |
| 20 |
Sureka, Neethi (11)
|
NTATAC |
40.17 |
47.14 |
6.97 |
| 21 |
Bouressa, Olivia N (11)
|
NTCOPS |
49.13 |
47.35 |
-1.78 |
| 22 |
Aswani, Sanaya S (12)
|
NTCOPS |
|
48.18 |
- |
| 23 |
Amberkar, Nidhi A (12)
|
NTCOPS |
48.54 |
49.22 |
0.68 |
| 24 |
Sain, Caroline E (12)
|
NTDM |
|
50.50 |
- |
| 25 |
Nowak, Alicja M (11)
|
NTCOPS |
|
59.27 |
- |
|
Burau, Allison A (12)
|
NTDM |
41.37 |
NS |
- |
|
Gonsalves, Aryna D (11)
|
NTCOPS |
|
NS |
- |
|
Escutia Castaneda, Ariadna (11)
|
NTCOPS |
47.33 |
NS |
- |