| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gehm, Kassidy A (14)
|
NIEMAC |
|
27.86 |
- |
| 2 |
Marshall, Olivia J (13)
|
NIEMAC |
29.06 |
28.88 |
-0.18 |
| 3 |
Stein, Shannen I (14)
|
NIEMAC |
28.48 |
28.90 |
0.42 |
| 4 |
Hurd, Taylor M (14)
|
NIGVST |
28.02 |
29.29 |
1.27 |
| 5 |
Steighner, Alexa M (14)
|
NIEMAC |
30.97 |
30.00 |
-0.97 |
| 6 |
Mikolajczyk, Emma R (13)
|
NIEMAC |
30.94 |
31.16 |
0.22 |
| 7 |
Edmister, Michaela R (14)
|
NIGVST |
|
33.00 |
- |
| 8 |
Stang, Hayley M (13)
|
NIGWST |
35.82 |
34.93 |
-0.89 |
| 9 |
Kerr, Aurora A (13)
|
NIPVSC |
34.55 |
36.33 |
1.78 |
| 10 |
Sternisha, Madison N (13)
|
NIGWST |
35.46 |
37.15 |
1.69 |
| 11 |
Oswald, Stephanie R (13)
|
NIGVST |
54.34 |
38.67 |
-15.67 |