| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wagner, Morgan E (10)
|
NIVICT |
2:33.31 |
2:30.66 |
-2.65 |
| 2 |
Reif, Rydell (10)
|
NISHAQ |
2:42.53 |
2:43.98 |
1.45 |
| 3 |
Reilly, Bridget F (10)
|
NISTAR |
2:52.94 |
2:48.21 |
-4.73 |
| 4 |
Golden, Lauren B (10)
|
NIBAAC |
2:58.81 |
2:59.35 |
0.54 |
| 5 |
Maas, Kaylee A (10)
|
NIIRON |
3:01.76 |
2:59.43 |
-2.33 |
| 6 |
Boyer, Chloe G (9)
|
NIISWM |
3:03.33 |
3:00.63 |
-2.70 |
| 7 |
Cook, Amelia A (10)
|
NIHAMB |
2:42.94 |
3:04.51 |
21.57 |
| 8 |
Choroser, Penelope T (10)
|
NITWST |
2:59.20 |
3:05.04 |
5.84 |
| 9 |
Sheehan, Ailise M (8)
|
NISEYS |
3:07.53 |
3:05.25 |
-2.28 |
| 10 |
Alessi, Luciana M (9)
|
NIBAAC |
3:19.09 |
3:06.78 |
-12.31 |
| 11 |
Roberts, Meredith J (9)
|
NITTSC |
3:08.90 |
3:07.63 |
-1.27 |
| 12 |
Barber, Cassandra L (10)
|
NIGRSC |
3:19.41 |
3:10.77 |
-8.64 |
| 13 |
Gilbert, Natalie J (9)
|
NIQUIK |
3:10.46 |
3:11.09 |
0.63 |
| 14 |
Kuznia, Arianna M (10)
|
NIJETS |
3:15.95 |
3:11.15 |
-4.80 |
| 15 |
Murphy, Campbell E (10)
|
NITTSC |
3:14.65 |
3:11.27 |
-3.38 |
| 16 |
Chen, Chloe Y (10)
|
NISDSC |
2:57.59 |
3:11.65 |
14.06 |
| 17 |
El Ghissassi, Sidra S (8)
|
NIVEST |
3:06.85 |
3:13.08 |
6.23 |
| 18 |
Crowley, Lila M (10)
|
NIGRSC |
2:53.97 |
3:13.17 |
19.20 |
| 19 |
Ashby, Emma M (9)
|
NISDSC |
2:56.44 |
3:24.69 |
28.25 |
| 20 |
Honaker, Addison R (9)
|
NICLSC |
3:03.24 |
3:24.76 |
21.52 |
| 21 |
Girard, Sydney V (10)
|
NIEAST |
2:52.33 |
3:36.59 |
44.26 |
| 22 |
Blakely-Armitage, Fiona S (9)
|
NIISWM |
3:50.36 |
3:40.66 |
-9.70 |