| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Moran, Aiden T (9)
|
NICLSC |
2:34.03 |
2:33.93 |
-0.10 |
| 2 |
Kabunov, Aleksei V (9)
|
NIJETS |
2:35.23 |
2:41.49 |
6.26 |
| 3 |
Lee, Aidan R (8)
|
NIUN-D |
2:43.58 |
2:43.46 |
-0.12 |
| 4 |
Anderson, Koen T (10)
|
NIISWM |
3:01.28 |
2:58.08 |
-3.20 |
| 5 |
Wakelee, Adam R (10)
|
NIVICT |
3:00.95 |
2:58.47 |
-2.48 |
| 6 |
Nishii-Kim, Issei H (10)
|
NIISWM |
3:03.75 |
2:58.76 |
-4.99 |
| 7 |
Cavallerano, Jack S (10)
|
NIUN-L |
3:06.38 |
2:59.34 |
-7.04 |
| 8 |
Chen, Ethan R (9)
|
NIUN-D |
2:59.32 |
2:59.66 |
0.34 |
| 9 |
Venkataswamy, Charan (10)
|
NIWAAC |
3:06.73 |
3:01.23 |
-5.50 |
| 10 |
Clark, Aidan J (10)
|
NIWAAC |
3:00.32 |
3:01.96 |
1.64 |
| 11 |
Kelley, Gage T (10)
|
NIFAST |
3:11.10 |
3:06.26 |
-4.84 |
| 12 |
Lorenz, Charles D (10)
|
NITTSC |
3:19.43 |
3:08.28 |
-11.15 |
| 13 |
Coen, Mitchell R (10)
|
NISTAR |
2:58.33 |
3:08.66 |
10.33 |
| 14 |
Ray, Gavin M (10)
|
NIVICT |
3:08.89 |
3:09.77 |
0.88 |
| 15 |
Parsnick, William Z (8)
|
NICLSC |
3:18.09 |
3:14.20 |
-3.89 |
| 16 |
Lewis, Christopher J (10)
|
NIQUIK |
3:00.47 |
3:14.47 |
14.00 |
| 17 |
Nienhaus, Matthew (9)
|
NISHAQ |
3:17.41 |
3:16.69 |
-0.72 |
| 18 |
Labib, Yassin (10)
|
NIUN-D |
3:09.76 |
3:20.23 |
10.47 |
| 19 |
Voit, Nicholas (10)
|
NIAMTS |
3:25.53 |
3:20.91 |
-4.62 |
| 20 |
Miller, Ian M (8)
|
NIIRON |
2:58.93 |
3:22.14 |
23.21 |
| 21 |
Elshamy, Yassine (9)
|
NIUN-D |
3:49.00 |
3:25.35 |
-23.65 |
| 22 |
King, Emmett M (9)
|
NITTSC |
3:20.69 |
3:28.34 |
7.65 |
| 23 |
Mangoba, Zachary A (10)
|
NIISWM |
3:39.55 |
3:43.09 |
3.54 |