| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Attuwaybi, Hamza B (10)
|
NIVELO |
46.11 |
44.48 |
-1.63 |
| 2 |
Kosikowski, Victor (10)
|
NIUN-D |
54.16 |
47.17 |
-6.99 |
| 3 |
Matteson, Cyrus L (10)
|
NISTAR |
47.85 |
47.75 |
-0.10 |
| 4 |
Marthi, Pranav C (10)
|
NIUN-D |
51.22 |
49.56 |
-1.66 |
| 5 |
Anderson, Max (9)
|
NIVELO |
50.87 |
50.01 |
-0.86 |
| 6 |
Venkataswamy, Charan (10)
|
NIWAAC |
51.59 |
50.71 |
-0.88 |
| 7 |
Wakelee, Adam R (10)
|
NIVICT |
51.37 |
51.80 |
0.43 |
| 8 |
Labib, Yassin (10)
|
NIUN-D |
51.56 |
52.54 |
0.98 |
| 9 |
Ray, Landon M (9)
|
NIVICT |
48.38 |
54.10 |
5.72 |
| 10 |
Crowther, Finn L (10)
|
NIVELO |
48.62 |
54.73 |
6.11 |
| 11 |
Voit, Nicholas (10)
|
NIAMTS |
55.57 |
55.00 |
-0.57 |
| 12 |
Olsen, Owen T (10)
|
NIAMTS |
57.30 |
56.60 |
-0.70 |
| 13 |
Battaglia, Benjamin P (9)
|
NIVICT |
53.71 |
57.34 |
3.63 |
| 14 |
Lewis, Christopher J (10)
|
NIQUIK |
53.21 |
57.58 |
4.37 |
| 15 |
Kelley, Gage T (10)
|
NIFAST |
47.90 |
57.73 |
9.83 |
| 16 |
Lenz, Keegan C (10)
|
NITWST |
53.05 |
58.15 |
5.10 |
| 17 |
Vitale, Nicolas K (10)
|
NIQUIK |
1:00.61 |
1:00.04 |
-0.57 |
| 18 |
Nienhaus, Matthew (9)
|
NISHAQ |
1:00.77 |
1:02.98 |
2.21 |
|
Hediger, Kobe K (10)
|
NIUN-E |
53.17 |
DQ |
- |
|
Clark, Ethan D (10)
|
NIMETS |
55.36 |
DQ |
- |