| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wahl, Zachary J (27)
|
NIWEBS |
54.16 |
57.43 |
3.27 |
| 2 |
Kreiley, Aidan C (15)
|
NIMERC |
57.12 |
58.94 |
1.82 |
| 3 |
Murphy, Liam (16)
|
NIPACK |
1:02.27 |
59.20 |
-3.07 |
| 4 |
Triaszin, Matthew V (17)
|
NIFAST |
57.61 |
59.62 |
2.01 |
| 5 |
Murphy, Brayden (16)
|
NIVICT |
58.03 |
1:00.17 |
2.14 |
| 6 |
LaRue, Carl V (15)
|
NIMERC |
58.93 |
1:00.23 |
1.30 |
| 7 |
Hall, Harrison J (13)
|
NIVICT |
1:02.84 |
1:00.92 |
-1.92 |
| 8 |
Smith, Connor J (14)
|
NIVICT |
1:04.57 |
1:01.28 |
-3.29 |
| 9 |
Semchenko, Alexander G (17)
|
NIUN |
|
1:02.59 |
- |
| 10 |
Dinh, Benson (14)
|
NIGRSC |
1:03.45 |
1:02.88 |
-0.57 |
| 11 |
Williams, Giovanni C (13)
|
NIPACK |
1:02.31 |
1:03.07 |
0.76 |
| 12 |
Searles, Tyler S (15)
|
NIIRON |
1:07.15 |
1:03.20 |
-3.95 |
| 13 |
Mortimer, Neil F (15)
|
NIPACK |
1:05.60 |
1:03.22 |
-2.38 |
| 14 |
Korshunov, Alexander V (13)
|
NISEYS |
1:00.61 |
1:03.32 |
2.71 |
| 15 |
Lang, Patrick J (13)
|
NISEYS |
1:01.44 |
1:04.46 |
3.02 |
| 16 |
DeMartinis, Ethan W (14)
|
NIMERC |
1:04.07 |
1:05.65 |
1.58 |
| 17 |
Markham, Rowan M (15)
|
NIFAST |
1:05.23 |
1:05.70 |
0.47 |
| 18 |
Staley, Nicholas R (14)
|
NIMERC |
1:03.78 |
1:07.13 |
3.35 |
| 19 |
Kreiley, Zackary D (13)
|
NIMERC |
1:10.95 |
1:09.61 |
-1.34 |
| 20 |
Palmer, Alexander R (13)
|
NIFAST |
1:15.26 |
1:13.17 |
-2.09 |
| 21 |
Dane, Samuel L (13)
|
NIVICT |
1:21.64 |
1:14.73 |
-6.91 |
| 22 |
Young, Gregory D (13)
|
NIMERC |
1:20.00 |
1:16.62 |
-3.38 |
| 23 |
Vitale, Christian R (13)
|
NIQUIK |
1:23.81 |
1:21.01 |
-2.80 |
| 24 |
Anthony, Jaydon T (13)
|
NIPMSC |
1:19.00 |
1:21.10 |
2.10 |
| 25 |
Pawloski, Andrew S (14)
|
NIFAST |
1:20.59 |
1:23.62 |
3.03 |
| 26 |
Monroe, Lucas J (12)
|
NIPMSC |
1:39.00 |
1:25.77 |
-13.23 |
|
Porretta, Gerald A (15)
|
NIIRON |
1:00.59 |
NS |
- |