| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Golembiewski, Lucas A (11)
|
NIPACK |
41.48 |
41.76 |
0.28 |
| 2 |
Gockeler Schultz, Jonathan S (12)
|
NIVICT |
|
43.11 |
- |
| 3 |
Sambhus, Laksh (12)
|
NIPACK |
57.54 |
43.86 |
-13.68 |
| 4 |
Schreyer, Jack E (10)
|
NISEYS |
50.44 |
44.35 |
-6.09 |
| 5 |
Lewis, Christopher J (10)
|
NIQUIK |
49.53 |
46.80 |
-2.73 |
| 6 |
Lewis, Matthew K (11)
|
NIQUIK |
43.10 |
47.50 |
4.40 |
| 7 |
Wakelee, Adam R (10)
|
NIVICT |
50.15 |
47.67 |
-2.48 |
| 8 |
Twohig, Colin M (10)
|
NIGRSC |
50.75 |
48.54 |
-2.21 |
| 9 |
Eagley, Wyatt T (10)
|
NIPMSC |
50.00 |
48.67 |
-1.33 |
| 10 |
Kelley, Gage T (10)
|
NIFAST |
|
48.88 |
- |
| 11 |
Frenzel, Jack W (10)
|
NIWEBS |
51.57 |
50.70 |
-0.87 |
|
Chasman, Aidan P (12)
|
NIVICT |
|
DQ |
- |
|
Harris, Wesley S (8)
|
NIVICT |
|
NS |
- |