| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Zhang, Ethan Y (10)
|
ADCPP |
45.09 |
38.91 |
-6.18 |
| 2 |
Striker, Aaron L (10)
|
ADCPP |
42.43 |
41.27 |
-1.16 |
| 3 |
Roglieri, Luca B (9)
|
ADCPP |
43.94 |
43.22 |
-0.72 |
| 4 |
Johnson, Aiden W (9)
|
ADWAVE |
52.59 |
47.13 |
-5.46 |
| 5 |
Sullivan, Conan P (10)
|
ADNTS |
51.80 |
48.58 |
-3.22 |
| 6 |
Thompson, Tehawennahkhwa (10)
|
ADNTS |
|
54.10 |
- |
| 7 |
Croniser, Gavin L (10)
|
ADTBS |
|
56.60 |
- |
| 8 |
Buckingham, Zane T (10)
|
ADTBS |
|
57.82 |
- |
| 9 |
Farnsworth, Bennett D (9)
|
ADWAVE |
1:03.98 |
58.46 |
-5.52 |
| 10 |
Houle, Perry J (9)
|
ADWAVE |
|
59.95 |
- |
|
Lagowski, Brian D (10)
|
ADCPP |
51.31 |
NS |
- |