| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Garrow, Ella A (12)
|
ADWAVE |
34.00 |
37.69 |
3.69 |
| 2 |
Law, Shelby L (12)
|
ADTBS |
|
38.41 |
- |
| 3 |
Myers, Myah K (12)
|
ADNTS |
37.59 |
38.89 |
1.30 |
| 4 |
Sheppard, Gillian M (12)
|
ADNHA |
44.00 |
39.98 |
-4.02 |
| 5 |
Thomas, Maya W (11)
|
ADNTS |
|
45.07 |
- |
| 6 |
Barkley, Katherine E (11)
|
ADNTS |
41.77 |
45.22 |
3.45 |
| 7 |
Anderson, Lillian M (12)
|
ADSVP |
51.53 |
49.36 |
-2.17 |
| 8 |
Andreescu, Stephanie (11)
|
ADNTS |
47.51 |
51.70 |
4.19 |
| 9 |
Pribble, Emily E (12)
|
ADNTS |
53.17 |
52.33 |
-0.84 |
| 10 |
Thompson, Hailey M (11)
|
ADNTS |
56.01 |
59.65 |
3.64 |
|
Betrus, Lindy J (11)
|
ADNTS |
50.22 |
DQ |
- |
|
Brooks, McKenna J (12)
|
ADNTS |
44.22 |
DQ |
- |
|
Shin, Jung E (11)
|
ADNTS |
|
DQ |
- |
|
Thornton, Kaydence L (11)
|
ADNTS |
|
DQ |
- |
|
Hlad, Emmilee C (12)
|
ADTBS |
|
DQ |
- |