| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pan-Wang, Evan (11)
|
NTLAC |
26.02 |
25.43 |
-0.59 |
| 2 |
Nguyen, Philippe D (12)
|
NTCOPS |
25.79 |
25.51 |
-0.28 |
| 3 |
Isaacs-Sodeye, Judah I (12)
|
NTNTN |
26.37 |
25.57 |
-0.80 |
| 4 |
Roybal, Hudson L (12)
|
NTLAC |
25.55 |
25.68 |
0.13 |
| 5 |
Beck, Jackson E (12)
|
NTLAC |
25.54 |
25.78 |
0.24 |
| 6 |
Silva, Carston S (12)
|
NTMAC |
25.55 |
25.79 |
0.24 |
| 7 |
Wang, Junxi (12)
|
NTUN |
27.31 |
26.04 |
-1.27 |
| 8 |
Wang, Ethan (12)
|
NTLAC |
26.43 |
26.25 |
-0.18 |
| 9 |
Heldenbrand, Dylan F (12)
|
NTNTN |
26.68 |
26.69 |
0.01 |
| 10 |
Ruhl, Jonah W (11)
|
NTATAC |
26.76 |
26.74 |
-0.02 |
| 11 |
Arangalla, Ramika A (12)
|
NTLAC |
26.88 |
26.79 |
-0.09 |
| 12 |
Agnihotri, Abhinav A (11)
|
NTLAC |
27.59 |
27.14 |
-0.45 |
| 13 |
Chu, Paul N (12)
|
NTCOPS |
27.64 |
27.19 |
-0.45 |
| 14 |
Chiang, Tyler B (12)
|
NTLAC |
26.53 |
27.30 |
0.77 |
| 15 |
Bender, Wesley F (12)
|
NTCOR |
27.59 |
27.67 |
0.08 |
| 16 |
Shippey, Drew T (12)
|
NTDM |
28.59 |
27.76 |
-0.83 |
| 17 |
Merrill, Elijah R (12)
|
NTRACE |
27.77 |
27.80 |
0.03 |
| 18 |
Lofthus, Jack W (11)
|
NTLAC |
28.43 |
28.64 |
0.21 |
| 19 |
Duran De La Vega, Pablo (12)
|
NTMTRO |
28.06 |
28.73 |
0.67 |
| 20 |
Wong, Alexander J (12)
|
NTLAC |
29.18 |
28.83 |
-0.35 |
| 21 |
Shih, Theodore K (12)
|
NTLAC |
30.49 |
30.20 |
-0.29 |