| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bonfiglio, Leah T (7)
|
VATIDE |
40.28 |
20.50 |
-19.78 |
| 2 |
Slivinski, Maeryn L (7)
|
VAODAC |
23.01 |
22.48 |
-0.53 |
| 3 |
Kubik, Alina R (7)
|
VAODAC |
24.43 |
23.50 |
-0.93 |
| 4 |
Zmuda, Kimberly N (7)
|
VAECAT |
31.18 |
23.97 |
-7.21 |
| 5 |
Wieck, Cailey J (7)
|
VAODAC |
27.46 |
24.38 |
-3.08 |
| 6 |
Wilkerson, Charlotte A (7)
|
VATIDE |
28.26 |
25.28 |
-2.98 |
| 7 |
Berdoulay, Ella K (7)
|
VAODAC |
|
27.40 |
- |
| 8 |
Krell, Riley A (7)
|
VAECAT |
25.28 |
27.74 |
2.46 |
| 9 |
Foxworth, Katherine E (7)
|
VAODAC |
35.28 |
32.92 |
-2.36 |
| 10 |
Embrey, Chloe F (7)
|
VATIDE |
|
35.50 |
- |
| 11 |
Ryan, Katelyn A (7)
|
VAODAC |
|
41.78 |
- |
| 12 |
MacDonaugh, Teaghan A (7)
|
VATIDE |
|
46.78 |
- |