| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Swartzbaugh, Anna M (6)
|
VAODAC |
30.58 |
28.97 |
-1.61 |
| 2 |
Sprague, Julianne G (6)
|
VATIDE |
|
29.23 |
- |
| 3 |
Stimson, Mia Y (6)
|
VAECAT |
|
34.25 |
- |
| 4 |
Mai, Elyse L (5)
|
VATIDE |
|
48.50 |
- |
|
Elhefnawy, Salma W (6)
|
VAECAT |
|
DQ |
- |
|
Fitzkee, Scarlett L (6)
|
VATIDE |
40.63 |
DQ |
- |
|
Hetzler, Noelle S (5)
|
VATIDE |
|
DQ |
- |
|
Korte, Willie D (5)
|
VATIDE |
|
DQ |
- |
|
Maisenbacher, Amalie K (6)
|
VATIDE |
|
DQ |
- |
|
Marshall, Megan T (6)
|
VATIDE |
|
DQ |
- |
|
Niederberger, Keira G (5)
|
VATIDE |
45.25 |
DQ |
- |
|
Cruz, Chrystine J (6)
|
VATIDE |
|
NS |
- |
|
Juergens, Mia A (6)
|
VATIDE |
|
NS |
- |